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Image Search Results
Journal: bioRxiv
Article Title: Curcumin Promotes Myelin Repair after Spinal Cord Injury via Spatially Selective Regulation of CLASP2 Phosphorylation
doi: 10.64898/2026.01.21.700776
Figure Lengend Snippet: (A) Schematic representation of study design. In thorax vertebrae 8-9 region of mice, 1 mm thick of spinal cord tissue was excised, and penicillin was injected muscularly daily for 3 days post-surgery. The groups were assigned to receive PBS, with the middle-dose group receiving 200 mg/kg/day and the high-dose group receiving 400 mg/kg/day. (B) BMS scores in mice rear legs of diverse groups. Values = means ± SEM. One-way analysis of variance (ANOVA) was conducted to examine the differences between groups, n=3 for each group. Con vs. CM-M, # p <0.05, ## p <0.01; Con vs. CM-H, * p <0.05, ** p <0.01, *** p <0.001. (C) Representative pictures of the hindlimb gait of control (complete spinal cord transection injury group), and treat (medium-dose group and high-dose group of curcumin). (D) Probability distribution chart of ankle dorsifiexion angles in each group. (E) Fibrotic scar and glial scar in the injured area of different groups. Immunostaining of tissues with chicken anti-GFAP (green) antibodies and mouse anti-Laminin antibodies (red). Nuclei were stained with DAPI. Scale bar represent 5 μm. (F) The myelin sheath and glial scar in the injured area of different groups. Immunostaining of tissues with chicken anti-GFAP (green) antibodies and mouse anti-MBP antibodies (red). Nuclei were stained with DAPI. Scale bar represent 5 μm. (G) Area of the fibrous scar defined by the fibrous scar marker Laminin from (E). Values = means ± SEM. One-way ANOVA, n=3 for each group. * P <0.05, ** P <0.01, n.s P> 0.05. (H) Area of the glial scar defined by the glial scar marker GFAP from (E). Values = means ± SEM. One-way ANOVA, n=3 for each group. n.s P> 0.05. (I) Integrated density of myelin sheath defined by MBP from (F). Values = means ± SEM. One-way ANOVA, n=3 for each group. * P <0.05, n.s P> 0.05.
Article Snippet: Avertin (Sigma-Aldrich, T48402-25G), penicillin sodium (FeiyuBIO, FY20311), Curcumin (Solarbio, C7090), NaOH (SCR, 1310-73-2), Na 2 HPO 4 (SCR, 7558-79-4), NaH 2 PO 4 (SCR, 13472-35-0), NaCl (SCR, 7647-14-5), Paraformaldehyde (SCR, 30525-89-4), Sucrose (SCR, 57-50-1), Triton-X-100 (Aladdin, T109026), O.C.T compound (Sakura, 4583), Tris (Solarbio, T8060), HCl (SCR, 7647-01-0), Ethylene Diamine Tetraacetic Acid (SCR, 60-00-4), NP-40 (Thermo Scientific, 28324), Na 3 VO 4 (Sigma-Aldrich, S6508-10G), NaF (SCR, 7681-49-4), Na 4 P 2 O 7 (Sigma-Aldrich, 7722-88-5), β-glycerophosphate(Sigma-Aldrich, 154804-51-0), Phosphatase inhibitor cocktail (Sigma-Aldrich, 524633), Complete Protease Inhibitor Cocktail (Roche, CO-RO), sodium deoxycholate (Sigma-Aldrich, V900388-50G), acetic acid (SCR, 64-19-7), sodium acetate (SCR, 127-09-3), zinc acetate (SCR, 5970-45-6), Phos-tag Agarose (Wako, 302-93561), L-Glutathione reduced (Sigma-Aldrich, 70-18-8), Nonfat dry milk (Beyotime, P0216), ECL kit (PerkinElmer, 122799-10), Tween-20 (Solarbio, T8220), TEMED (Amresco, 110-18-9), 30% Acrylamide (Solarbio, A1010), ammonium persulfate (SCR, 7727-54-0), methyl alcohol(SCR, 67-56-1), Fetal bovine serum (VivaCell, C04001-050), P/S (Pricella, PB180120), Bovine serum albumin (AMRESCO, 9048-46-8), Typsin (Sigma-Aldrich, R00950), Poly-L-lysine (Yeasen, 60716ES08), AntiFade Reagent (Sigma-Aldrich, P36930-2), FluorSave Reagent (Millipore, 345789), Laminin (Abcam, IF 1:1000, ab133645),
Techniques: Injection, Control, Immunostaining, Staining, Marker